Granuloma inguinale (donovanosis) is diagnosed with which stain?

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Prepare for the NBME Microbiology Test. Study with flashcards and multiple-choice questions. Each query comes with hints and detailed explanations. Ace your exam!

Multiple Choice

Granuloma inguinale (donovanosis) is diagnosed with which stain?

Explanation:
Granuloma inguinale is diagnosed by identifying Donovan bodies inside macrophages from lesion scrapings. These intracellular, bipolar-staining inclusions of Klebsiella granulomatis are best visualized with a Giemsa stain, which provides good contrast and highlights the characteristic appearance. Gram staining isn’t reliable for these intracellular bodies, and acid-fast staining targets mycobacteria rather than this organism. Wright stain can show Donovan bodies, but Giemsa is the classic choice for diagnosing this infection.

Granuloma inguinale is diagnosed by identifying Donovan bodies inside macrophages from lesion scrapings. These intracellular, bipolar-staining inclusions of Klebsiella granulomatis are best visualized with a Giemsa stain, which provides good contrast and highlights the characteristic appearance. Gram staining isn’t reliable for these intracellular bodies, and acid-fast staining targets mycobacteria rather than this organism. Wright stain can show Donovan bodies, but Giemsa is the classic choice for diagnosing this infection.

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